acc 99

Subculture: seed out at ca. 99 candi dalam 1 malam 0 5-1 x 10 6 cells/ml; it might be of advantage to start culture initially in a 12- or 24-well-plate; after thawing viability may decrease to about 50% and possibly no cell growth during the first 3-4 days is ibserved (do not dilute); later on maintain at 0 7-1 5 x 10 6 cells/ml and split growing culture 1:2 to 1:3